Naa10p Inhibits Beige Adipocyte-mediated Thermogenesis through N-α-acetylation of Pgc1α

We reported that both conventional and adipose-specific Naa10p deletions in mice result in increased energy expenditure, thermogenesis, beige adipocyte differentiation and activation. Mechanistically, Naa10p acetylates the N-terminus of Pgc1α and prevents it from interacting with Ppar𝛾 to activate key genes, such as Ucp1, involved in beige adipocyte function. We used microarrays to profile the gene expression changes by Naa10p KO in inguinal white adipose tissues (iWATs) derived from mice fed with high fat diet for 15 weeks. Complementary DNAs were generated with 3 μg of total RNAs extracted from iWATs of 3 WT and 3 Naa10-KO mice fed with HFD for 15 weeks and exposed to 4°C for 4 hrs, and subsequently hybridized with Gene Chip Mouse Genome 430 2.0 Array.