EGF and TGF-β1 biochemical factor release profiles from AM substrates preserved using different techniques.

Preparations of AM were cultured over a 10 day period in PBS, using Scaffdex supports. Samples of PBS were taken at different time points and EGF (A) and TGF-β1 (B) levels were measured by ELISA. Release profiles demonstrate an immediate and time dependent release of both EGF and TGF-β1 from all of the AM substrates, over the 10 day culture period. Cryopreserved AM showed a significant increase (*p<0.05) in EGF release at days 4 and 7 compared to the sustained release from the remaining substrates at the equivalent time points. Conversely raffinose treated AM demonstrated a significant increase (*p<0.05) in TGF-β1 release at days 4, 7 and 10 compared to cryopreserved AM and the remaining substrates. Data are expressed as mean ± SEM based on six separate experiments.