Derivative solute concentrations in various dilutions of the EAFS10/10 vitrification solution.

Columns D–G. The molalities are calculated as Columns N-Q of Table 2/MW of the respective solute.Columns H–K: The molarities are calculated as 100× Column B/Wt% in Column G-K of Table 2/MW of the solute.Column L: The nonpermeating solutes are sucrose and PBS (we ignore the very small contribution of the Ficoll and BSA.) Their combined osmolality is Column F+0.276. We assume the osmolality of PBS to be equal to that of the same molality of NaCl. The osmolality of NaCl = 2φm where 2 is the number of species into which the molecule dissociates and φ is the osmotic coefficient.Column M: The volume of water in the oocytes after equilibration with the external medium relative to the volume of water in an isotonic cell. It is 0.276/Column L.