Identification of the role of tumor intrinsic Malat1 in regulation of immune microenvironment [scRNA-seq]

We have injected 4T1-TGL breast cancer cells into Balbc mice through tail vein. In order to see immune cells infiltration, we have isolated CD45+ cells from these mice lungs at different time points by FACS. These isolated cells were further subjected for scRNA-seq. For base line we have isolated CD45+ cells from Naive balbc mice. Overall design: Lung CD45+ cells were isolated for scRNA-seq and sent for sequencing at MDACC Sequencing Core Facilities. The sample name was annotated below. SC78_1: Naïve SC78_2: WT-D10 SC78_3: KO-D10 SC78_6:WT-D20 SC78_7: KO-D20 SC78_9: WT-D30 SC78_10: KO-D30