Next Generation Sequencing Facilitates Quantitative Analysis of Ocular Adnexal B-cell Lymphoma Transcriptomes

Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to describe NGS-derived transcriptome profiling (RNA-seq) of ocular adnexal B-cell lymphomas (OABLs). Gene expression profiling of RNA was performed on OABL samples to identify pathway alterations and expression changes affected by clinical traits mRNA profiles of 31 heterogenous samples from OABL patients were generated by deep sequencing using Illumina Novaseq 6000 according to the manufacturer's direction. RNA was extracted from cryopreserved lymphoma tumor specimens. The sequence reads that passed quality filters were analyzed at the transcript isoform level.