RNA-Seq of murine lung endothelial cells stimulated with IFN-gamma and dexamethasone

Glucocorticoids are potent anti-inflammatory drugs prescribed in various diseases such as asthma, rheumatoid arthritis and multiple sclerosis. The effects of glucocorticoids have extensively been studied in immune cells. However, the regulation of inflammation by glucocorticoids in endothelial cells remains poorly understood. In this study, we stimulated murine lung endothelial cell line with dexamethasone (100 nM) and IFN-gamma (20 ng/mL) for 24 hours and extracted RNA for RNA-seq.