Rates of ura4 loss (including genomic deletion, translocation, and mutation events), calculated using the method of the median.

a the following nomenclature is used: “t” and “ori” refer to the telomere and the replication origin 3006/7, respectively; “” indicate the RTS1-RFB and its polarity ( blocks replication forks moving from the telomere towards the origin 3006/7).b event/cell/division ×10−8 ± standard error. Values are means of at least 3 independent rates.c statistical significance was determined using the nonparametric Mann-Whitney U test.