Pesticide protein discovery platform and insecticidal proteins found therefrom

The present invention provides a platform for discovering novel pesticidal proteins from highly heterogeneous environmental sources. This methodology utilizes a metagenome enrichment procedure and a unique gene amplification technique to provide access to a wide variety of unknown microbial diversity and the resulting proteome. The published pesticidal protein discovery platform (IPDP) can be computationally driven and integrate molecular biology， automation and advanced machine learning protocols. The platform allows researchers to quickly and accurately access a vast repertoire of untapped pesticidal proteins produced in uncharacterized and complex microbial environmental samples. In addition， the present invention provides a group of newly discovered pore-forming toxins (PFTs) in rare types of pesticidal proteins discovered using pesticidal protein discovery platforms. The disclosure presents a platform for discovering novel insecticidal proteins from highly heterogeneous environmental sources. The methodology utilizes metagenomic enrichment procedures and unique genetic amplification techniques， which enables access to a broad class of unknown microbial diversity and their resultant proteome. The disclosed insecticidal protein discovery platform (IPDP) can be computationally driven and is able to integrate molecular biology， automation， and advanced machine learning protocols. The platform will enable researchers to rapidly and accurately access the vast repertoire of untapped insecticidal proteins produced by uncharacterized and complex microbial environmental samples. Also presented herein are a group of newly discovered pore-forming toxins (PFT) from a rare class of insecticidal proteins， which were discovered utilizing the insecticidal protein discovery platform. The disclosure presents a platform for discovering novel insecticidal proteins from highly heterogeneous environmental sources. The methodology utilizes metagenomic enrichment procedures and unique genetic amplification techniques， which enables access to a broad class of unknown microbial diversity and their resultant proteome. The disclosed insecticidal protein discovery platform (IPDP) can be computationally driven and is able to integrate molecular biology， automation， and advanced machine learning protocols. The platform will enable researchers to rapidly and accurately access the vast repertoire of untapped insecticidal proteins produced by uncharacterized and complex microbial environmental samples. Also presented herein are a group of newly discovered pore-forming toxins (PFT) from a rare class of insecticidal proteins， which were discovered utilizing the insecticidal protein discovery platform.