Genome-wide mapping of H3K27ac by Cut&Tag in CD4+ and CD8+ immune cells of astronauts following prolonged orbital space flight.

Cut&Tag for H3K27ac was performed in isolated CD4+ and CD8+ immune cells of astronauts undergoing prolonged orbital space flight and in control subjects. Blood samples for astronauts were collected at the following time points: 55 – 185 days before flight (pre-flight, PF), within the first 24 h after landing (R+1) and 20 – 50 days after landing (R+35±15). Blood draw for control subjects was performed at the following time points: at the beginning of the study (PF), after 251 days (R+1) and 20-50 days after the R+1 blood draw (R+35±15). CD8+ and CD4+ immune cells were isolated from blood of astronauts and control subjects and subjected to Cut&Tag for H3K27ac.