Expression profiling by high throughput sequencing to evaluate the effect of aroylated phenylenediamine HO53 on autophagy induction in mature human bronchial epithelial cells (BCi-NS1.1 cell line)

We used RNA sequencing to exploit molecular mechanisms behind HO53 induced autophagy in mature human bronchial epithelial cells (BCi-NS1.1 cell line). We performed transcriptome analysis of mature bronchial epithelial cells (BCi-NS1.1 cell line) treated with aroylated phenylenediamine HO53 for different time points 4 h, 8 h and 24 h and compared it to transcriptome of untreated cells collected at the same time points. A broad effect of HO53 on the gene expression in mature human lung epithelial cells (BCi-NS1.1) shows molecular signature for pathways involved in autophagy regulation. The expression pattern for selected genes was reconfirmed by qRT-PCR using SYBR Green reagent. Overall design: Expression profiling by high throughput sequencing of HO53 treated vs untreated differentiated in air-liquid intephase (ALI) culture human bronchial epithelial cells (BCi-NS1.1 cell line)