Cisplatin-inducible death receptor 5 antisense long non-coding RNA modulates cell cycle and proliferation in HeLa cells

We have performed a transcriptomics study in which we first transfected LNA GapmeRs against DR5-AS lncRNA to silence it in HeLa cells. Total RNA was isolated from control as well as transfected cells and silencing was confirmed by qPCR. Total RNAs were subjected to deep-sequencing to identify differentially expressed mRNAs. We then took advantage of bioinformatic tools to identify which pathways are affected by DR5-AS knock-down. Total RNA was isolated from negative control (Negative GapmeR) and DR5-AS GapmeR treated HeLa cells in triplicate to perform deep-sequencing. Negative GapmeR was used as control and denoted as 160701_SND393_B_L002_GDU-31, 160701_SND393_B_L002_GDU-32 and 160701_SND393_B_L002_GDU-33