A CRISPR/Cas9 strategy to generate DNA replication- repair compromised fluorescent cellular models of MMR deficient childhood cancers
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下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA690957
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资源简介：
CRISPR/Cas gene editing technologies has enabled rapid generation of disease models.Removal of DNA mismatches by the mismatch repair pathway is crucial for replication fidelity and survival of dividing cells. MMR deficiency has been linked to colorectal, endometrial and childhood cancers. To obtain clues into the function and regulation of mlh1 in DNA replication and repair, we used plasmid-based CRISPR/Cas9 technology to generate a mlh1 knockout in a fluorescently tagged osteosarcoma cell line, U2OS-H2B-GFP.
创建时间：
2021-01-09
