RNA-seq of postnatal day 0 (P0) wild-type and Fezf2-/- cortices

The goal of the study was to compare gene expression of P0 wild-type and P0 Fezf2-/- cortices. Total RNAs were isolated from P0 cortices dissected from wild-type and Fezf2-/- mice (n=3 for each genotype), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA TruSeq library preparation guide.The libaries were pair-end sequenced (50nt per end). Differentially expressed genes were identified by DESEQ. Total RNAs were isolated from P0 cortices (3 control and 3 mutants), and sequenced on Illumina Genome Analyzer本研究旨在比较出生后第0天（P0）野生型（wild-type）与Fezf2基因敲除型（Fezf2-/-）小鼠大脑皮层的基因表达水平。本研究依照凯杰（Qiagen）RNAeasy总RNA提取试剂盒的操作指南，从野生型与Fezf2基因敲除型小鼠的P0大脑皮层组织中提取总RNA，每组基因型设置3个生物学重复（n=3）。随后依照Illumina RNA TruSeq建库制备指南构建测序文库。上述文库采用双端测序模式进行测序，每端读取长度为50个核苷酸（50nt）。采用DESeq软件鉴定差异表达基因。另从P0大脑皮层组织中提取总RNA（3个对照样本与3个突变样本），并在Illumina基因组分析仪上完成测序。