Isotropic tissue expansion using ProteomEx

Figure 3. Validation of ProteomEx in different tissue types. (A) Representative bright-field images of different tissue slices pre-expansion (left column) and post-expansion (middle column; Coomassie-stained) and overlay (right column) of pre-expansion image (green pseudo-color) and registered post-expansion image (magenta pseudo-color). Arrows represent the deformation vector field (n=3, 3, and 3 tissue slices from one mouse each; in the images of the expanded sample white circles represent the dissected area for peptide analysis shown in C). (B) The root-mean-square (RMS) measurement length error for pre- versus post- expansion brain slice images for the experiments shown in A (n=3, 3, and 3 tissue slices from one mouse each; average LEF = 5.8, 6.5, and 6.2 for brain, liver, and tumor respectively; blue line, mean; shaded area, standard deviation (SD)). Computer codes used for isotropic analysis for this study are available from Github (https://github.com/Wendy-k2/Isotropy-quantification).