Expression profiles of OE (Overexpression of ANAC069) plants and KO (Knockout of ANAC069, SALK_095231C) plants under salt stress conditions

To identify the target genes regulated by ANAC069, the expression profiles of OE (Overexpression of ANAC069) plants and KO (Knockout of ANAC069, SALK_095231C) plants under salt stress conditions were compared using Affymetrix Arabidopsis gene chips. Four-week-old OE (OE-3 line) and KO (KO-2 line) plants were treated with 200 mM NaCl for 3 h. Then gene expression in OE and KO plants was compared using Affymetrix Arabidopsis gene chips (ATH1 Genome Array), performed with three independent biological replicates.