The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs

Drug hypersensitivity reactions (DHR) are commonly observed with various medications. However, some drugs, like Qingkailing (QKL), iodixanol, and vancomycin, previously classified as pseudoallergenic, do not align with clinical observations, creating uncertainty in understanding DHR mechanisms and implementing prevention. In response, our research team hypothesized that IgE-mediated allergy and pseudoallergic hypersensitivity may synergistically lead to more severe reactions. We established an IgE-mediated hypersensitivity model using an anti-dinitrophenyl IgE monoclonal antibody in RBL-2H3 cells and employed ovalbumen (OVA) for guinea pigs. Compound 48/80 served as a positive control for non-immunologic hypersensitivity. We measured β-hexosaminidase and histamine released in cells and guinea pigs to focus on hypersensitivity responses from both IgE-mediated and pseudoallergic pathways. Results indicated that combining pseudoallergenic drugs and IgE significantly increased the hypersens..., , <br />

# Data from: The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs

Dataset DOI: [10.5061/dryad.bvq83bkpz](https://doi.org/10.5061/dryad.bvq83bkpz)

## Description of the data and file structure

This dataset supports the research article titled \"The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs\" (PLOS ONE Manuscript ID: PONE-D-25-28449R2), authored by Yu Zhang, Chengbo Zheng, Qilong Xu, Cunyu Li, Yunfeng Zheng, and Guoping Peng.

### Files and variables

#### File: DATA_PONED2528449R2_YuZhang_20260124.xlsx

**Description:** In vitro experiments were conducted using RBL-2H3 cells, a widely used model for studying allergic responses. The dataset includes key measurements such as cell degranulation rates (assessed by β-hexosaminidase release assay), levels of inflammatory cytokines (e.g., IL-4, TNF-α) detected via ELISA, and cell viability data. These data reflect the respons...,