Extra-HEK293-AAV: Transcriptomics of modified HEK293 cell line for higher productivity of biologics (e.g. viral vectors, AAV)

The human EXT1 gene has been knockdown in HEK293 cells to improve their performance in producing viral vectors such as AAV. This work compares the gene expression for the wildtype and kd_EXT1 cells, in adherent and suspension cultures. After 5 passages, cells were trypsinized (for adherent culture only) and collected by centrifugation. RNA was extracted using the NucleoSpin RNA extraction kit from Macherey-Nagel. RNAseq was performed with an Illumina NovaSeq X Plus. Mapping was done with the reference GRCh38 and the gene expression level analysis was calculated.