Expression data from splenocytes - Wild type Myd88 or mutated MYD88-L252P mice

The MYD88 L265P mutation, activator of NF-kappa B (NF-kB), is found in 80% to 90% of WM. So far, there is no existing animal model for WM to evaluate the role of MYD88 activation, and the only published mouse model harboring the mouse ortholog MYD88 L252P mutation develops aggressive B-cell lymphomas in aged mice. The MYD88 L252P coding sequence was attached to YFP cDNA with an IRES sequence. This construct was flanked with loxP sites and inserted in the Rosa26 locus. The expression of MYD88 L252P protein is induced only in B cells after crossing with CD19_Cre mice. Total mRNA was extracted from whole spleen tissues. Amplification of RNAs and hybridization onto microarrays were performed on an Affymetrix Gene Atlas system® with the MoGene-2_1-st-v1 Affymetrix chip (Affymetrix, Santa Clara, CA) as described (Chanut A et al, Leukemia 2013).