sec10MO embryos show variable gross phenotypes at 3 dpf.

Abnormal gross phenotypes, scored at 3 dpf, included any of the following: smaller body size, small eyes, edema, and curly tail down and curly tail up. Lysates isolated from normal- and abnormal-appearing 3 dpf sec10MO embryos showed similar levels of zfSec10 knockdown (data not shown). The following Fisher's exact tests were performed on the proportions of wild-type embryos: First, examination of the total percentages of wild-type phenotypes in all three conditions showed that the frequency of wild-type embryos differed across conditions (p-15). Second, examination of the 15ng sec10MO embryos compared to the 8+8ng sec10MO embryos showed that the latter yielded a significantly higher frequency of wild-type embryos (p