Phylogenomic insights and geographic distribution of the New World genus Amphibolips Reinhard (Hymenoptera: Cynipidae, Cynipini) using ultraconserved elements

AbstractIn recent years, new wasp species and genera of Cynipidae have been described, and their species delimitation and evolutionary relationships have been supported using molecular markers. However, few studies have included comprehensive and extensive sampling of specimens across the complete distribution of a single genus. In this study, we analysed the phylogenetic relationships of the genus Amphibolips throughout its range using ultraconserved elements (UCEs). We collected 520 adult wasps from 401 sites in various geographic locations, predominantly in Mexico, and used 78 wasps for UCE analysis. Our results demonstrate that Amphibolips forms a monophyletic clade. Amphibolips can be further subdivided into four genetically well‐differentiated clades. Each clade had morphological traits that could be collectively characterised. Species delimitation using molecular sequencing highlights the taxonomic complexity involved in separating and assigning species using exclusively morpholo..., , , # Phylogenomic insights and geographic distribution of the New World genus Amphibolips Reinhard (Hymenoptera: Cynipidae, Cynipini) using ultraconserved elements (UCEs)

[https://doi.org/10.5061/dryad.3ffbg79tk](https://doi.org/10.5061/dryad.3ffbg79tk)

### Files

#### File: Phylogenomics\_of\_New\_World\_Amphibolips.zip

**Description:** This folder contains three folders inside.

The first of these folders is called `alignments`. It contains the alignments in phylip and nexus format for each data matrix used during the analyses.

The second folder is called `individual_uce_trees_passed_symmetry_test`. It contains two individual tree files of UCEs that passed the symmetry test in IQ-TREE. These files are needed to use molecular species delimitation with SODA.

The third folder is called `trees_supplementary_material`. It contains the .tree files of the trees that are left as supplementary material in the manuscript.

**Abbreviations used for file names:**

* Amphi: Refers to the *Amphib...