Stokes radii observations and predictions for the expressed regions of NS1-2.

1The observed Stokes radii (RS) from the Superose12 size exclusion column (SEC) were determined from the standard curve (r2 = 0.9820) obtained after calibration of the column with the standards from the Sigma MW-GF-200 kit.2The RS value from dynamic light scattering (DLS) is the average value ± SD obtained from five (NS1-2trunc monomer) and three (NS1-2trunc dimer and NS1-2casp) independent measurements.3The predicted RS values were calculated for each possible protein conformation from the equations described in the Hydrodynamic characterizationmethods section. NF, natively folded. MG, molten globule. PMG, premolten globule. UN, unfolded in urea.