TET1 and 5-hydroxymethylation preserve the stem cell state of mouse trophoblast

Analysis of 5mC and 5hmC and associated transcription in trophoblast stem cells cultured and differentiated at 20% and 5% oxygen, integrated with previously published datasets Overall design: meDIP and hmeDIP was carried out to determine 5mC and 5hmC genomic distribution in tcontrol rophoblast stem cells and those lacking Tet1. hmeDIP and RNA-seq was also carried out in trophoblast stem cells cultured in 5% Oxygen to determine the effect of hypoxia on 5hmC and associated transcription