Ballistic Microscopy (BaM) data repository

Light and electron microscopy utilizes interactions of either photons or electrons with matter to create images from cellular to atomic scale. However, these methods are limited in de novo discovery and spatial mapping of unknown biomolecules. Label-free methods, such as mass spectrometry or sequencing, lack live-cell and subcellular context. Here, we introduce a new approach, Ballistic Microscopy (BaM), to image cells with physical nanoparticles. We bombard living cells with millions of nanoparticles traveling at ∼1000 m/s. Each particle passes through cells, piercing and capturing attoliters of cytoplasm on a hydrogel substrate while preserving spatial information (SPLAT-MAP). This “physical image” of a live cell captures a molecular fingerprint of a cell on a hydrogel film that can be processed post-capture via multiple techniques such as TEM, Cryo-EM, mass spectrometry, confocal imaging, and DNA amplification. Using BaM, we discover a previously unknown composition of CLIP170 and Ta..., , # Ballistic Microscopy (BaM) data repository

Dataset DOI: [10.5061/dryad.70rxwdcc1](https://doi.org/10.5061/dryad.70rxwdcc1)

## Description of the data and file structure

The contents of this dataset were used in the associated preprint \"Ballistic Microscopy (BaM).\" It includes multiple data, including confocal fluorescence microscopy, transmission electron microscopy (TEM), cryo-electron microscopy (cryo-EM), high-speed imaging, mass spectrometry data from CLIP-170 condensates, and protein gel images.

This dataset contains several types of data used in the manuscript. File names are organized and labeled according to main figures, supplementary figures, videos, and supplementary tables.

.xlsx files can be opened using Microsoft Excel; .mrc and .tif files can be opened using ImageJ/Fiji; .cys files can be opened using Cytoscape; and .stl files can be opened using PrusaSlicer.

## **01 Table S2. List of proteins in CLIP170 condensates.pdf**

* 01_Table_S2._List_of_proteins_in_CLIP17...,