1,3-Dipolar Cycloaddition Reactions for the Synthesis of Novel Oxindole Derivatives and Their Cytotoxic Properties

The multicomponent reaction between
isatin, amino acid, but-2-ynedioates,
and phenacyl bromide has been developed using microwave irradiation
under catalyst and base-free conditions in aqueous medium. This synthetic
protocol is useful for the synthesis of various functionalized spirooxindole
derivatives. This MCR exhibits a broad substrate scope with excellent
yields and shorter reaction time. Additionally the synthesized spirooxindole
derivatives were evaluated for their anticancer activity against three
human cancer cell lines: MCF-7 (breast), A549 (lung), and HeLa cervical.
Most of the compounds showed moderate to potent cytotoxic activity
against the tested cell lines.