Basal and stimulated activities of wild type and HNOX mutants in COS-7 cells.

Specific activities (spec. act.) are expressed in pmol. min−1. mg−1 of cytosolic fraction ± SEM and were measured from 2 to 3 independent transfections. Each assay under each condition was repeated three times and in duplicate. Fold stimulation (fold st) strongly lower or higher than WT is in italic and in bold, respectively. Cytosolic fractions were prepared as described in Material and Methods.