RNA sequencing analysis of gene expression in mouse ventral hippocampus exposed to chronic stress. Mus musculus

The ventral hippocampus from control and CUMS treated mice was dissected for RNA sequencing. Total RNA was extracted using TRIzol Reagent (Invitrogen, 15596026) according to the protocol provided by the manufacturer . Construction of the cDNA library and sequencing were performed by Guangzhou RiboBio Co., Ltd. (China). Clean reads were obtained after discarding reads containing adapter, poly-N, or low quality raw data. High-quality reads were aligned to the mouse reference genome mm10 with HISAT2. Next, HTseq was used to convert aligned short reads into read counts.