Concordance of IgE detection by allergen microarray and ELISA.

Specific IgE was measured for six allergens in 40 human sera using ELISA, an assay commonly used for clinical diagnosis. Cytoplasmic fractions and commercial extracts of each allergen were assayed. We scored each serum as positive (+) or negative (−) for specific IgE to each allergen by ELISA and allergen microarray using empirically defined detection thresholds (Methods). The results are cross-tabulated here, showing the counts of assays positive by one technique, positive by both techniques, and negative by both techniques (proportion of total assays in parentheses).