Next Generation Sequencing Quantitative Analysis of Wild Type and TMEM9 KD Transcriptomes. Next Generation Sequencing Quantitative Analysis of Wild Type and TMEM9 KD Transcriptomes

Purpose: The goals of this study are to check signaling pathway change upon TMEM9 KD using NGS-derived retinal transcriptome profiling (RNA-seq). Methods: Retinal mRNA profiles of MDA-MB-453 WT and TMEM9 KD were generated by deep sequencing, using Illumina. The sequence reads that passed quality filters were analyzed. Results: Using GSEA analysis, we found mTOR signaling pathway was suppressed after TMEM9 KD. Conclusions: Our study revealed the signaling pathway change upon TMEM9 KD in BRCA cells. Overall design: Retinal mRNA profiles of MDA-MB-453 cells