Expression data from human HMGN4- and HMGN2-expressing mouse embryionic fibroblasts

HMGN proteins, a family of non-histone nucleosome-binding proteins, maintain a dynamic balance between more and less compacted fibers via interactions with other architectural elements of chromatin. This balance affects the ability of regulatory factors to access their target sites, which eventually modifies the expression profile of the cell. We used microarrays to detail gene expression change in mouse fibroblasts overexpressing human HMGN4 to identify outlier genes and distinct classes of up- and down-regulated genes during this process. Mouse embryonic fibroblasts (MEFs), MEFs, expressing hHMGN4 (MEF-N4) or hHMGN2 (MEF-N2) or vector alone (MEF-V, served as a control) were harvested from exponentially grown cell culture for RNA extraction and hybridization on Affymetrix microarrays.