Effect of cabozantinib on transcriptome and signaling pathways in leukemic cell line Kasumi-1

To identify molecular effects of the tyrosine kinase inhibitor cabozantinib (XL184), we applied gene expression profiling on human acute leukemic cell lines Kasumi-1, which harbors t(8;21) coupled with KIT mutation, by Whole Transcriptome Shotgun Sequencing (RNA-seq). After Kasumi-1 cells were treated with 100nM cabozantinib for 4h or 24h, differentially expressed genes (DEGs) of 124 genes upregulated and 416 were downregulated in 4h, and 184 genes upregulated and 69 genes were downregulated in 24h treatment, compared with vehicle group. Gene expression in Kasumi-1 was measured after exposure for 4h or 24 h to 100nM cabozantinib (XL184) or to the vehicle control (DMSO).