Bulk RNA-seq of Normal and Doxorubicin-Induced Senescent HCA2 cells

Purpose: Use RNA-seq strategy to characterize the PD-L1 correlated genes in sorted PD-L1 negative and positive doxorubicin-induced senescent and normal human foreskin fibroblasts HCA2 cells to identify the upstream transcriptional network for regulating heterogeneous PD-L1 expression in senescent cells. Methods: Early passaged normal HCA2 cells and senescent HCA2 cells induced by 24 hours of 100 nM doxorubicin treatment were utilized for the preparation of bulk RNA-seq libraries. Among the senescent cells, PD-L1 positive and negative cells were separated by FACSorting. Overall design: Examination of differentially expressed genes and PD-L1 correlated genes within sorted PD-L1 positive and negative d-Sen HCA2 cells.