Arabidopsis thaliana cultivar:Col Transcriptome or Gene expression. Arabidopsis thaliana cultivar:Col

Although large-scale transcriptome analyses of iron deficiency in the whole shoots have been investigated, detailed information of how different leaves respond to Fe deficiency remains uncharacterized. To dissect processes that are triggered by Fe deficiency, we conducted in-depth spatial-transcriptional profiling experiments from different-pair leaves. The first (largest), second (middle) and third (smallest) pair leaves at different positions from Fe-sufficient and Fe-deficient plants were separately collected and subjected to RNA-seq.