Kisspeptin photometry recordings during pregnancy and lactation

The specific role that prolactin plays in lactational infertility, as distinct from other suckling or metabolic cues, remains unresolved. Here, deletion of the prolactin receptor (Prlr) from forebrain neurons or arcuate kisspeptin neurons resulted infailure to maintain normal lactation-induced suppression of estrous cycles. Kisspeptin immunoreactivity and pulsatile LH secretion were increased in these mice, even in the presence of ongoing suckling stimulation and lactation. GCaMP fibre photometry of arcuate kisspeptin neurons revealed that the normal episodic activity of these neurons is rapidly suppressed in pregnancy and this was maintained throughout early lactation. Deletion of Prlr from arcuate kisspeptin neurons resulted in early reactivation of episodic activity of kisspeptin neurons prior to a premature return of reproductive cycles in early lactation. These observations show dynamic variation in arcuate kisspeptin neuronal activity associated with the hormonal changes of pregna..., GCaMP fibre photometry recordings of arcuate nucleus kisspeptin neurons throughout pregnancy and lactation. 
Fluorescence signals were acquired using a custom-built fibre photometry system made primarily from Doric components. Violet (405nm) and blue (490nm) fibre-coupled LEDs were sinusoidally modulated at 531 and 211 Hz, respectively, and focused into a 400μm, 0.48 numerical aperture fibre optic patch cord connected to the mouse. Emitted fluorescence was collected by the same fibre and focused onto a femtowatt photoreceiver (2151, Newport). The two GCaMP6s emission signals were collected at 10 Hz in a scheduled 5s on/15s off mode by demodulating the 405nm (non-calcium dependent) and 490nm (calcium-dependent) signals. The power output at the tip of the fibre was set at 50μW. Fluorescent signals were acquired using a custom software acquisition system (Tussock Innovation, Dunedin, New Zealand) and analyzed using custom templates created by Dr Joon Kim (University of Otago, Dunedin, New ..., , # Kisspeptin photometry during pregnancy and lactation

[https://doi.org/10.5061/dryad.8931zcs21](https://doi.org/10.5061/dryad.8931zcs21)

## Description of the data and file structure

These files contain the data used to construct the figures in the associated published manuscript in eLife. Data files contain processed data from GCaMP fiber photometry recordings (in .csv format), and in some cases plasma LH (luteinizing hormone) concentrations.  The raw data have been processed as described in the manuscript, and are provided here as ΔF/F(%) against time. Plasma LH concentrations are available at a limited number of time points and are provided as ng/ml. Each filename starts with a code number (F##) which is a unique identifier for each animal in the study (e.g. female number ##).  In most cases, there are multiple recordings from an individual animal collected at different times across pregnancy and lactation.  These are provided as separate files and are sequentially numbered wit...