Long-read RNA sequencing of cattle, pig, and chicken tissues

Long-read Nanopore cDNA sequencing of polyA-enriched RNA was implemented in a range of adult tissues isolated from cattle, pig, and chicken. These data were used to identify and characterize the expression patterns of full-length transcript isoforms. Overall design: RNA was extracted from a total of 189 biological samples, corresponding to 93 cattle samples (32 tissues from two males and two females; represented as one 'Cattle_tissues_mixed' sample), 28 pig samples (17 tissues from two males), and 68 chicken samples (19 tissues from four males and two females). Raw data were procured from two lanes of PromethION sequencing: one lane for pooled cattle libraries, and one lane for pooled pig and chicken libraries. Please note that 'Cattle_tissues_mixed' sample represents non-demultiplexed cattle samples.