Spontaneous food leaving from an OP50 lawn without pairing with 2-nonanone.

Wild type, mutant animals and transgenic animals are examined for food leaving for 1 hour. Young adult worms are placed on an OP50 food lawn for 10 minutes and the number of worms on food lawn is counted every 5 minutes for a total assay time of 60 minutes. The percentage of worms off the food lawn at 15 minutes is reported. n = 2–4 assays for each genotype and 20–25 worms in each assay, mutants or transgenic animals are compared with wild-type animals tested in parallel with student’s t test, Mean ± SEM.