Gene expression profile of sorted microglia and CD206 border-associated macrophages obtained from mice

Brain macrophages have long been considered a homogenous population as determined by flow cytometry. We use scRNAseq to investigate the significant pheotypic heterogeneity of brain macrophages, especially under neurodegenerative disorders, based on their transcriptional profile. Overall design: Microglia and CD206+ border-associated macrophages were isolated from 6-month-old healthy and APP(NL-G-F) mice, with FIT2-WT or FIT2-Mut, respectively, to study their transcriptome alteration during Alzheimer's disease progression.