Metadata record for the article: MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer

Summary This metadata record provides details of the data supporting the claims of the related article: “MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer”. The related study aimed to determine how triple negative breast cancer (TNBC) suppresses anti-tumour macrophages by using microRNA-200c (miR-200c), a powerful repressor of epithelial-to-mesenchymal transition (EMT), to drive mesenchymal-like mouse mammary carcinoma and human TNBC cells towards a more epithelial state. Type of data: mRNA sequencing Subject of data: Mus musculus; Eukaryotic cell lines; antibodies Sample size: When appropriate, tissue culture experiments are the mean of three separate experiments conducted in triplicate to decuple. For in vivo experiments, animal numbers were calculated at 80% power to the expected difference (based on published studies) at P Population characteristics: 6-8 week old female FVB/NJ mice (IMSR Cat# JAX:001800, RRID:IMSR_JAX:001800) Data access The mRNA sequencing data are openly available in the Gene Expression Omnibus via the following accession: https://identifiers.org/geo:GSE151320. The majority of the other data files generated as part of the related study are openly available as part of this data record. A comprehensive list of which data underlie which element of the related article is available as part of this data record in the file ‘Williams_et_al_2021_underlying_data_list.xlsx’. Other data used in the related study are already openly available in various repositories, and access details are also given in the file mentioned above. Data supporting generation of Met-1 TripZ-200c and Met-1 TripZ-EV cells are contained in the files ‘Cell sort RPF- 011818.pdf’ and ‘Cell sort RFP+ 020918.pdf’. Corresponding author(s) for this study Jennifer K Richer, Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO USA. [e-mail]. Study approval All animal experiments were performed in accordance with international, national and institutional guidelines for humane research under a protocol (#00407) approved by the University of Colorado Institutional Animal Care and Use Committee (IACUC).