SV2AB and CIP data

ATP hydrolysis reactions were conducted at 25°C with purified recombinant SV2-FLAG or commercially available calf intestinal alkaline phosphatase (CIP). An aliquot of CIP was heated at 95 °C for 20 min to inactivate the enzyme.
At designated intervals (0 min, 1 min, 2 min, 5 min and 10 min), aliquots were removed from the reaction for analysis by the malachite green assay described as above. Duplicates were assayed for each time point and the average value of the duplicates was used for quantification.

在25°C的条件下，对经过纯化的重组SV2-FLAG或市售的小牛肠碱性磷酸酶（CIP）进行了ATP水解反应。将CIP的一部分在95°C下加热20分钟以灭活酶活性。在指定的时间间隔（0分钟、1分钟、2分钟、5分钟和10分钟）内，取出反应混合物的一部分，按照上述所述的孔雀绿法进行检测。每个时间点均进行重复检测，并以重复检测的平均值进行定量。