Gene expression in mature and immature human ES-derived beta cells, and sorted beta-cells from adult islets

This submission comprises RNA-Seq profiling of in vivo differentiated pancreatic beta cells types and primary islet-derived pancreatic beta cell sources. The study reports that re-aggregation of immature beta-like cells (d20Beta) and removal of progenitor cells leads to further maturation in vitro resulting in beta-cells (eBCBeta) that are closer to human primary islet-derived beta-cells(IsletBeta). As a control stem cell derived spheres were dissocated, reassociated and taken through till the end of the protocol (NECBeta). Overall design: 2 samples each of eBCBeta, NECBeta, d20Beta and IsletBeta were for RNA-seq by using SMARTer Stranded Total RNA Sample Prep Kit – Low Input Mammalian Kit (Clontech) kits for library preparation after RNA integrity was confirmed. Sequencing was carried out on the Illumina platform