BoDV-1 infection during neuronal differentiation

Borna disease virus (BoDV-1) is a highly neurotropic RNA virus, which causes neurobehavioral disturbances such as abnormal social activities and memory impairment. In this study, we investigated the effects of BoDV-1 infection on neuronal differentiation and transcriptome of differentiated neuronal cells using persistently BoDV-1-infected SH-SY5Y (SB) cells. Differentiated neuronal cells exhibited transcriptomic changes of genes related to neurite outgrowth and antiviral immune system. SH-SY5Y (SHSY) and BoDV-1-infected SH-SY5Y (SB) cells were treated with 10 µM retinoic acid (RA) for 2 days. Total RNA was isolated from the cells and subjected to RNA-seq analysis.