RNA sequencing of of an Arctic moss Aulacomnium turgidum depending on cold acclimation and freezing tolerance

Cold acclimation refers to a phenomenon in which plants become more tolerant to freezing after exposure to non-lethal low temperatures. Aulacomnium turgidum is a moss found in the Arctic and can be used as an important material for studying freezing tolerance of bryophyte. To improve our understanding of cold acclimation effect on freezing tolerance of A. turgidum, we compared the electrolyte leakage of protonema grown at 25C, non-acclimation, NA and at 4C, cold acclimation, CA and found that freezing damage was significant reduced in CA at -12C. For the comparative analysis of transcriptome between NA-12 and CA-12, six cDNA libraries were constructed in triplicate and RNA-seq reads were obtained.