ChIP-on-chip of Cdc45 in S. pombe. ChIP-on-chip of Cdc45 in S. pombe

ChIP-on-chip of Cdc45 in synchronized cells operating with the analog-sensitive CDK fusion protein (Cdc13-Cdc2). Cells were released from a G2 block or from a G2 block followed by an extended G1 (G1+ 15 min extension). For the G2 block, samples were collected 30 minutes after release. For the G1+15 extension, samples were collected 3 minutes after release. Timepoint of collection was based on qPCR experiments showing Cdc45 binding at origins of replication. Overall design: Each IP was compared to its reciprocially labeled input sample. Duplicates for every condition