Single-cell RNA-sequencing of proximal colon epithelial cells from Gpr35 knock-out mice

We performed a single-cell transcriptome analysis of proximal colon epithelial cells from 4 Gpr35 KO and 4 WT female littermates mice at a steady state. Crypts were dissociated to single-cell suspensions sorted for CD326+, CD45- and CD31- cells, and the eight samples were loaded onto the wells of a single 10X Genomics Chromium controller.