Classification of TE evolutionary dynamics

We conducted an analysis of the breakpoints of nonautonomous copies and determined the number of nonautonomous copies with breakpoints occurring at the same position or within a 50-bp vicinity to account for issues such as alignment uncertainty. Nonautonomous elements with at least three copies at the same position were defined as a MITE. For active DNA TEs, the distribution of MITEs could be classified into three scenarios or models: the majority of nonautonomous copies are MITEs; TEs lack MITEs, and the nonautonomous copies demonstrate random internal deletions, leading to length heterogeneity; and in a hybrid scenario, one or more MITEs are present, but the majority exhibit random deletions.