Use of the copepod Oithona hebes as a bioencapsulator of essential fatty acids

AbstractThe advantages of using copepods in aquaculture include nutritional superiority, high digestibility and broad spectrum of sizes, with the possibility of bioencapsulation of nutrients, probiotics and medicines. This study aimed to compare the effects of feeding copepods with a microalgae diet and two commercial inert diets on the copepod culture performance and their fatty acid profile. Wild copepods were collected in the estuarine system of Piraquê-açu River, Aracruz, Espírito Santo, Brazil, with a conical net of 60 cm in diameter and 200 μm mesh with a blind cup end, towed through the subsurface layer at a speed of 1 knot for 5 minutes. Once collected, the material was sieved in order to select only Oithona hebes. The experiment was conducted in nine cylindrical-conical tanks with a 60 L capacity, salinity of 25.8 ± 1.3, temperature of 25.5 ± 0.5 ºC and weak aeration, stocked with a density of 1.5 copepod/mL. Treatments were made in triplicate and consisted of: Treatment 1 (Control) fed with microalgae Chaetoceros gracilis and Nannochloropsis oculata (1:1) with 50,000 cells.mL-1each; Treatment 2 with S.Parkle® INVE (1g.million-1); and Treatment 3 with freeze-dried spirulina (1g.million-1). The mean final population was compared by a Tukey test (p