Alternative scaffolds for de novo whole genome sequencing of the eastern brown snake, Pseudonaja textilis.

The eastern brown snake, Pseudonaja textilis, has a potent venom and is one of the most deadly snakes in Australia. We have performed whole genome sequencing and de novo assembly of the eastern brown snake, using 10x Chromium linked reads. Supernova v2 assembly of the data generated a 1.59 Gb draft genome with a maximum scaffold size of 55.1 Mb, primary scaffold N50 of 14.7 Mb (2.47% gaps) and a primary scaffold BUSCO (v2.0.1 short) completeness estimate of 90.5% (brown snake). Pseudodiploid processing of the assembly generated non-redundant alternative scaffolds for 1.23 Gb (77.6%) of the assembly, with an N50 of 17.1 Mb (2.38% gaps).