Detection of δRec-ψJa T-cell receptor excision circles in macrophage cultures.

aAdherent cells from Donors 914 and 898 were harvested from primary macrophage cultures at day 38 and 42, respectively, using accutase, and replated in 24-well plates. For the donor 225 cultures, nonadherent cells recovered from day 13 primary cultures were plated at low density in T150 flasks, and then thirteen days later, the adherent cells were harvested and replated in 24-well plates. Adherent cells from donors 895 and 898 were harvested from primary cultures at day 26, depleted of CD3+ cells, and then replated in 24-well plates;
bnonadherent cells were recoverable by washing, loosely adherent cells were primarily T-cells that required short-term accutase treatment for detachment, and adherent cells were macrophages;
cthe “0” values shown indicate that no TREC were detected;
dthis value represents an approximate average value among different assays. The TREC values shown represent the mean of replicate samples ± standard deviation.