Effect of Inflammatory cytokines primed hUC-MSCs therapy on gene expression of macrophages in IUA-like mice model

Intrauterine adhesion (IUA), characterized by endometrial fibrosis, is a common cause of female reproductive disorders. IUA-like mice model was created with curettage plus LPS stimulation to mimic endometrial fibrosis in IUA patients. Mesenchymal stem cells (MSCs) based therapy is applied to some refractory immune diseases to control inflammation and their immunomodulatory function can be enhanced via pre-treatment with inflammatory cytokines. In this work, we examined the effect of untreated and cytokines primed MSCs therapy on macrophage in IUA-like mice model. The uteri samples were collected from sham, IUA modeling, IUA+control MSCs treatment and IUA+primed MSCs treatment groups of mice. Endometrial macrophages were sorted through F4/80 magnetic beads and performed RNA-seq. Comparative gene expression profiling analysis of RNA-seq data for macrophages sorted from mice uteri with 3 sham operation (Sham), 3 IUA modeling (Matrigel), 3 IUA+control MSCs treatment (Ctrl) and 3 IUA+primed MSCs treatment (Primed).