Lactuca sativa var. angustana Raw sequence reads. Lactuca sativa var. angustana

The deterioration in the quality of fresh-cut asparagus lettuce (Lactuca sativa var. angustata) during storage can be significantly delayed by immersing it in a solution containing 0.67 % sodium carboxymethyl cellulose (CMC-Na), 0.38 % ascorbic acid (AsA), and 0.85 % L-cysteine (L-Cys), resulting in improved color and texture maintenance, as well as an extended shelf life. Transcriptomic analysis was used to elucidate the mechanism by which the solution delays the deterioration of fresh-cut asparagus lettuce. This demonstrated that the accelerated deterioration of fresh-cut asparagus lettuce was primarily attributed to enhanced respiration and transpiration after mechanical injury, along with increased susceptibility to microbial infection. In comparison to a control group, treatment with the solution effectively attenuated the upregulation of the LsACO3 and LsACS1 genes; it downregulated the expression of the phospholipase-related genes LsNPC6, LsPLC2, and At2g42690. Conversely, there was substantial upregulation of gene expression related to L. sativa calmodulin-like proteins and L. sativa calcium-dependent protein kinases, along with activation of the MAPK signaling pathway and significant upregulation of genes associated with L. sativa ethylene-responsive transcription factors involved in the defense response. These results indicate that treatment with the solution effectively delays the deterioration of fresh-cut asparagus lettuce during storage by inhibiting endogenous ethylene synthesis, mitigating reactive oxygen damage, and enhancing disease resistance, thereby providing superior preservation of its color and texture.