RNA-seq of Arabidopsis seedlings treated with chemical modifiers of sugar signalling at dawn

Arabidopsis seedlings were treated with DPI, pentamidine isethionate, tyrphostin AG879, AEG3482 or DMSO (control) at dawn (ZT0). Samples were collected for mRNA-seq for untreated seedlings at ZT0 or treated seedlings at ZT2. 14-day-old Columbia-0 (Col-0) Arabidopsis thaliana seedlings grown on 1/2MS in 12 h light/12 h dark cycles transferred to 1/2MS media at ZT0 (before lights on) containing 0.1% (v/v) DMSO, 10 µM DPI, 25 µM pentamidine isethionate, 2 µM Tyrphostin AG879 or 12.5 µM AEG3482. Untreated samples were collected at ZT0 and treated samples were collected at ZT2 in triplicate.